Bzr1 chip-seq
WebThe function of BZR1 on organ size is well characterized in Arabidopsis, but poorly understood in maize (Zea mays). ... ChIP and RNA-seq analysis showed ZmBZR1 can directly bind to the promoter region of organ size related genes, Germination Repression and Cell Expansion receptor-like kinase (GRACE) and KIP-RELATED PROTEIN6 … WebJul 22, 2012 · Among the six PRE members, PRE1, PRE5 and PRE6 were identified as PIF4 targets in the ChIP-seq analysis (Supplementary Fig. S6a), and confirmed by ChIP–qPCR to be direct targets of both BZR1 and ...
Bzr1 chip-seq
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WebDec 3, 2024 · In silico analysis showed that the promoters PILS2, PILS3, and PILS5 are direct targets of BZR1 and BES1 (BZR2) and the binding of BZR1 to PILS2 and PILS5 … WebOct 7, 2024 · The bri-t-like phenotype of bzr-h suggested that BR signaling was completely shut off in the bzr-h mutant. To obtain evidence to support this conclusion, we treated 2 …
WebFeb 9, 2024 · Transcriptome profile of BZR1-responsive genes during seed germination via RNA-seq. To explore other BZR1-responsive targets involved in regulating rice germination, ... (2008) with minor modifications. In brief, young panicles of BZR1-OX. ChIP-qPCR was performed as described previously (Tong et al., 2014). WebApr 3, 2024 · Because BZR1 and its closest homolog BES1 act as key transcription factors downstream of BIN2 in the BR-signaling pathway (He et al., 2002, Yin et al., 2002), we examined the freezing tolerance of their dominant active mutants, bzr1-1D and bes1-D.The bzr1-1D and bes1-D mutants showed increased freezing tolerance compared with the …
WebJan 3, 2024 · A bioinformatics analysis of PHR1 targets identified by chromatin immunoprecipitation sequencing (ChIP-seq) revealed that ARF7 and ARF19 are targets of PHR1, thereby raising the possibility of a regulatory feedback loop formed between PHR1 and ARF7 and ARF19 (Crombez et al., 2024). WebJan 26, 2024 · In this chapter, the RNA-seq and ChIP-seq protocols to identify BR target genes are described and an overview of the methods for data analyses is presented as …
Web总 RNA 提取和 qRT-PCR 分析\RNA-Seq. Western Blot\重组蛋白纯化和 EMSA\染色质免疫沉淀 (ChIP)-qPCR. 双荧光素酶测定\酵母单杂交试验 ... ( C ) WT 植物、 bzr1和hy5突变体侧芽中BRC1的转录水平。 ...
WebIdentification of BZR1 direct target genes using ChIP-chip Source publication Integration of Brassinosteroid Signal Transduction with the Transcription Network for Plant Growth Regulation in ... gray stain for kitchen cabinetsWebAug 13, 2024 · We generated a first-generation (T 0) transgenic CRISPR-bzr1 line. PCR genotyping and sequencing identified a deletion of 42 bp in proximity to single guide RNA (sgRNA) target sequences ... (E, F) ChIP-qPCR assay of BZR1 binding to the promoters of FER2 and FER3 genes in control and heat stress conditions. gray stain for hardwood floorsWebSep 13, 2024 · To test whether BES1 and BZR1 can directly regulate the expression of DYT1, we carried out a chromatin immunoprecipitation (ChIP) analysis to investigate whether BES1 and BZR1 can be enriched in ... gray stain for wood cabinetsWebJan 20, 2024 · Once significant human genome sequencing began for the HGP, a 'draft' human genome sequence (as described above) was produced over a 15-month period … cholelithiasis and psylliumWebChromatin immunoprecipitation (ChIP) is a powerful method for studying TF-DNA interactions in vivo. To identify all binding sites of a TF in the genome, the DNA obtained in ChIP experiments needs to be analyzed by hybridization to genome-tiling microarrays (ChIP-chip) or by next-generation sequencing (ChIP-seq). gray stain for wood furnitureWebTo generate comparable data sets, we performed BZR1 ChIP-115 Seq analysis using dark-grown seedlings (Figure 1-source data 1B), as used in the ARF6 and 116 PIF4 ChIP-Seq (Oh et al., 2012). Interestingly, large portions of ARF6 binding targets 117 overlapped with either targets of BZR1 (51%) or PIF4 (71%) or both (42%) (Figure 1C). The cholelithiasis and vomitingWebLists of BR- and auxin-responsive genes and BZR1 target genes. Comparison of genes up- or down-regulated by BR or auxin, identified by RNA-Seq in roots (Table 2-S1), to the BZR1 ChIP-chip and ChIP-Seq data [S1, S2], and the RootMap spatial gene expression data [S3]. QC, quiescent center; MZ, meristem zone; TEZ, transition-elongation zone; LEZ ... gray stain for wood